Tm Calculators:
NEB – Tm Calculator (Preferred)
ThermoFisher – Tm Calculator
Optimize your PCR reaction using the Gradient PCR
Optimizing PCR Primer’s Tm and Annealing Temperatures
Articles
Primer Validation Was a No-Go – Now What?
Annealing Temperature of 55°C and Specificity of Primer Binding in PCR Reactions
